Generating haploid plants for the purpose of obtaining pure double haploid (DH) lines is widely recognized as one of the most efficient methods of breeding in modern agriculture. Watermelon (Citrullus lanatus), an important fruit plant known for its nutrients and taste, has undergone long-term artificial selection resulting in genetic narrowing. Therefore, there is an urgent need for a haploid induction system to improve traditional breeding methods and facilitate the development of valuable pure DH lines.
In March 2023, the Plant Biotechnology Journal published “Production of double haploid watermelon by maternal haploid induction” by Xu Yong’s team from the Beijing Academy of Agricultural and Forestry Sciences. This report shows the successful production of DH plants by in vivo (seed-based) haploid induction system, a pioneering success in cucurbit plant breeding. It introduces a new method for the implementation of the haploid breeding system within the Cucurbitaceae family.
Coincidentally, in April 2023, the journal Horticultural Research also published a paper titled “Generation of a watermelon haploid inducer line by ClDMP3-mediated single fertilization of the central cell” by Yuan Li’s team at the College of Horticulture, Northwest A&F University.
Previous studies have shown that irradiation of pollen grains can induce haploids in cucurbit plants, probably due to the preferential fertilization of the central cell rather than the egg cell. It is known that the destruction of the DMP gene can cause a fertilization of the central cell, which leads to the development of haploid plants.
This new study of Horticultural Research provided a detailed procedure for creating a watermelon haploid inducer line by ClDMP3 mutation. The cldmp3 mutant successfully induced haploid plants in several watermelon genotypes at a rate reaching up to 1.12%. These haploid plants were confirmed by using fluorescent markers, flow cytometry, molecular markers, and immuno-staining. The creation of this haploid inducer line has great potential for significant improvement in watermelon breeding in the future.
Shouwei Tian et al, Production of double haploid watermelon by maternal haploid induction, Plant Biotechnology Journal (2023). DOI: 10.1111/pbi.14045
Xiner Chen et al, Generation of a watermelon haploid inducer line by ClDMP3-mediated single fertilization of the central cell, Horticultural Research (2023). DOI: 10.1093/hr/uhad081
Provided by NanJing Agricultural University
Citation: Researchers create double-haploid watermelon plants by in vivo, seed-based haploid induction system (2023, July 10) retrieved on July 11, 2023 from https://phys.org/news/2023 -07-double-haploid-watermelon-vivo -seed-based-haploid.html
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